HDF-n are isolated from neonatal human skin. HDF-n are cryopreserved at P0 and delivered frozen. Each vial contains > 5 x 105 cells in 1 ml volume. HDF-n are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HDF-n are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HDF-n are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium: It is recommended to use Fibroblast Medium (FM, Cat. no. SC2301) for culturing HDF-n in vitro.
Product Use: HDF-n are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.