Components: FM-sf-prf consists of 500 ml of basal medium, 5 ml of fibroblast growth supplement-2 (FGS-2, Cat. No. SC2362), and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. SC0503).
Product Use: FM-sf-prf is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Store the basal medium at +4oC, the FGS-2 and the P/S solution at -20oC. Protect from light.
Shipping: Gel ice. Prepare for use Thaw FGS-2 and P/S solution at +37oC. Gently tilt the FGS-2 tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add FGS-2 and P/S solution into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of this medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed +37oC. When stored in the dark at +4oC, the reconstituted medium is stable for one month.