Quality Control: • Purity: A260/A280 ratio of 1.9-2.1• RNA integrity: intact 18S rRNA bands and 28S rRNA bands verified by a denaturing agarose gel, with a 2:1 ratio of 28S:18S.
Application:• RT-PCR and Real Time RT-PCR• cDNA synthesis• RNA protection assay• Northern blot analysis• Purification of mRNA for library construction• PCR-select cDNA subtraction
HDF-n tRNA is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Store the RNA immediately at -80°C upon arrival. To ensure stability, it is recommended to aliquot the RNA into single-use fractions to avoid repeated freeze/thaw cycles.