BDF are isolated from fetal bovine skin. BDF are cryopreserved at passage one and delivered frozen. BDF are characterized by immunofluorescence with antibody specific to fibronectin. BDF are negative for mycoplasma, bacteria, yeast, and fungi. BDF are guaranteed to further expand for 5 population doublings under the conditions provided.
Recommended Medium
It is recommended to use Fibroblast Medium-2 (FM-2, Cat. No. SC2331) for culturing BDF in vitro.
Product Use
BDF are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] Gabbiani G, Rungger-Brandle E. (1981) “The fibroblast.” In Glynn LE, Handbook of Inflammation, Vol. 3: Tissue Repair and Regeneration (pp 1-50). Amsterdam: Elsevier. [2] Conrad GW, Hart GW, Chen Y. (1977) “Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks.” J Cell Sci. 26: 119-37. [3] Boo S, Dagnino L. (2012) “Integrins as modulators of transforming growth factor beta signaling in dermal fibroblasts during skin regeneration after injury.” Adv Wound Care. 2(5): 238-246.