Macrophages are cells differentiated from circulating bone marrow-derived monocytes. In the bone marrow and subsequestly in the blood and tissues, monocytes undergo a cascade of maturation that culminate in morphologically and functionally mature tissue macrophages [1]. Macrophages function to removal cellular debris and invading pathogens. They phagocytize invading microorganisms, and scavenge dead, damaged cells and cellular debris. Macrophages can be identified by the specific expression of several cell surface proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), MAC-1/MAC-3, and CD68 by flow cytometry or immunohistochemical staining [2].
Recommended Medium
It is recommended to use Macrophage Medium (MaM, Cat. No. SC1921) for the culturing of RMa-bm in vitro.
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1]. Martin Cline and Margaret Sumner (1972) Bone Marrow Macrophage Precursors. I. Some Functional Characteristics of the Early Cells of the Mouse Macrophage Series Blood 40(1) 62-69.
[2]. Siamon Gordon and Philip Taylor (2005) Monocyte and macrophage heterogeneity. Nature Reviews Immunology 5(12) 953-964.