Macrophages are cells differentiated from circulating bone marrow-derived monocytes. Their main function is to remove cellular debris and invading pathogens. Hepatic macrophages, which are also known as Kupffer cells, reside within the lumen of liver sinusoids. Hepatic macrophages protect the liver by responding to pathogens and metastatic cells, whilst tolerating harmless self and foreign antigens, which enter via blood flow through portal vein and hepatic artery [1]. Recent studies have shown that hepatic macrophages play an important role in fibrosis, liver inflammation, alcoholic liver disease, fatty liver disease, liver transplantation and more [2-5]. Macrophages isolated from fresh rat liver tissue serve as an excellent model for studying their functions under normal physiological and pathological conditions.
Recommended Medium
It is recommended to use Macrophage Medium (MaM, Cat. No. SC1921) for culturing RHMa in vitro.
Product Use
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1]. Wynn TA and Barron L. (2010). Semin Liver Dis. 30:245-57.
[2]. Liaskou E, Wilson DV and Oo YH. (2012). Mediators Inflamm. 2012: 949157.
[3]. Ajakaiye M, Jacob A, Wu R, Zhou M, et al. (2011). Biochem Biophys Res Commun. 409: 406-411.
[4]. Bieghs V, Verheyen F, van Gorp PJ, Hendrikx T, et al. (2012) PLoS One. 7: e34378.
[5]. Tian Y, Jochum W, Georgiev P, Moritz W, et al. (2006). Proc Natl Acad Sci U S A. 103: 4598-4603.
[6]. Seki E, De Minicis S, Inokuchi S, Taura K, et al. (2009). Hepatology. 50: 185-197.
[7]. Gordon S and Taylor P. (2005). Nature Reviews Immunology. 5:953-964.