Macrophages are cells differentiated from circulating bone marrow-derived monocytes. The main function of macrophages is to remove cellular debris and destroy invading pathogens. Mouse Hepatic Macrophages (MHMa), which are also known as Kupffer cells, reside within the lumen of liver sinusoids. MHMa protect the liver by responding to pathogens and metastatic cells, while tolerating harmless self and foreign antigens, which enter via blood flow through the portal vein and hepatic artery . Recent studies have shown that hepatic macrophages play an important role in fibrosis, liver inflammation, fatty liver disease, and liver transplantation [2-4]. MHMa are an excellent model for studying macrophage functions under normal physiological and pathological conditions.
It is recommended to use Macrophage Medium (MaM, Cat. No. SC1921) for culturing MHMa in vitro.
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
Liaskou E, Wilson DV, Oo YH. (2012) "Innate immune cells in liver inflammation."Mediators Inflamm. 2012: 949157. Bieghs V, Verheyen F, van Gorp PJ, Hendrikx T, Wouters K, Lutjohann D, Gijbels MJ, Febbraio M, Binder CJ, Hofker MH, Shiri-Sverdlov R. (2012) "Internalization of modified lipids by CD36 and SR-A leads to hepatic inflammation and lysosomal cholesterol storage in Kupffer cells."PLoS One. 7: e34378. Tian Y, Jochum W, Georgiev P, Moritz W, Graf R, Clavien PA. (2006) "Kupffer cell-dependent TNF-alpha signaling mediates injury in the arterialized small-for-size liver transplantation in the mouse." Proc Natl Acad Sci U S A. 103: 4598-603. Seki E, de Minicis S, Inokuchi S, Taura K, Miyai K, van Rooijen N, Schwabe RF, Brenner DA. (2009) "CCR2 promotes hepatic fibrosis in mice." Hepatology. 50: 185-97