Macrophages are cells differentiated from circulating bone marrow-derived monocytes. In the bone marrow and subsequestly in the blood and tissues, monocytes undergo a cascade of maturation that culminate in morphologically and functionally mature tissue macrophages . Macrophages function to removal cellular debris and invading pathogens. They phagocytize invading microorganisms, and scavenge dead, damaged cells and cellular debris. Macrophages can be identified by the specific expression of several cell surface proteins including CD14, CD11b, F4/80 (mice)/EMR1 (human), MAC-1/MAC-3, and CD68 by flow cytometry or immunohistochemical staining .
It is recommended to use Macrophage Medium (MaM, Cat. No. SC1921) for the culturing of Mouse Macrophages from C57BL/6 in vitro.
Mouse Macrophages from C57BL/6 are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
ClineMJ,SumnerMargaret A.(1972) "Bone Marrow Macrophage Precursors. I. Some Functional Characteristics of the Early Cells of the Mouse Macrophage Series."Blood.40: 62-9. Gordon S, Taylor P. (2005) "Monocyte and macrophage heterogeneity."Nature Reviews Immunology.5: 953-64.