Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen [1]. There is evidence showing that fibroblasts in different organs are intrinsically different [2]. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli [3].
Recommended Medium
It is recommended to use Fibroblast Medium-2 (Cat. No. SC2331) for culturing Mouse Dermal Fibroblasts in vitro.
Product Use
Mouse Dermal Fibroblasts are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
Warranty
Cells are only warranted if ScienCell media and reagents are used and the recommended protocols are followed.
References
[1] Gabbiani G, Rungger-Brandle E. (1981) "The fibroblast." In Glynn LE, Handbook of Inflammation, Vol. 3: Tissue Repair and Regeneration (pp 1-50). Amsterdam: Elsevier. [2] Conrad GW, Hart GW, Chen Y. (1977) "Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks." J Cell Sci. 26: 119-37. [3] Stair S, Carlson KW, Shuster S, Wei ET, Stern R. (2002) "Mystixin peptides reduce hyaluronan deposition and edema formation." Eur J Pharmacol. 450: 291-6.