RNA preparation: The total RNA is prepared using modified guanidinium method. The RNA has been treated with RNase-free DNase I and dissolved in RNase free water.
Quality control:
• RNA quality of A260/280 ratio was in the range 1.9-2.1 RNA Intergrity number (RIN) >8, was determined using an Agilent 2100 Bioanalyzer.
• Concentration of RNA was determined using Nanodrop spectrophotometer.
• cDNA synthesis was tested by RT-PCR.
Application: Total RNA is ready-for-use in cDNA synthesis, RT-PCR, RNA protection assay, northern blot analysis, purification of mRNA for library construction, PCR-select cDNA subtraction and microarray.
Storage: -80˚C
Shipping: Dry-ice or in ethanol solution at room temperature.