MIF, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 12.5 kDa. The MIF is purified by proprietary chromatographic techniques.
Synonyms: Macrophage migration inhibitory factor, MIF, Glutathione-binding 13 kDa protein, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase.
Formulation: Lyophilized from a 0.2 µm filtered concentrated solution in 1×PBS, pH 7.4 and 5% trehalose.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized MIF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized MIF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MIF should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Source:Escherichia coli. Amino acid sequence: MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA.
Uniprot ACC#: P30904
Transportation method: Shipped at Room temperature
Safety data sheet: Inquire at info@3hbiomedical.com