Leptin, Rat Recombinant, produced in
Escherichia coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16 kDa. The Leptin is purified by proprietary chromatographic techniques.
Synonyms: OB Protein, Obesity Protein, OBS, Obesity factor.
Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Solubility: It is recommended to reconstitute the lyophilized Leptin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: Biological activity of Leptin Rat is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-His.
Uniprot ACC#: P50596
Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin rat as a Reference Standard.
Transportation method: Shipped at Room temperature
References: 1. Title:Expression of Hypothalamic KiSS-1 System and Rescue of Defective Responses by Kisspeptin in Streptozotocin-Induced Diabetic Male Rats. Publication: doi: 10.2337/db05-1584 Diabetes September 2006 vol. 55 no. 9 2602-2610 Link:http://diabetes. diabetesjournals. org/content/55/9/2602. full?patientinform-links=yes&legid=diabetes;55/9/26022. Title:Desensitization of responses to kisspeptin in the female rat: analyses of LH and FSH secretion at different developmental and metabolic states. Publication:Am J Physiol Endocrinol Metab 294: E1088–E1096, 2008. 294: E1088–E1096, 2008. First published April 15, 2008; doi:10.1152/ajpendo. 90240.2008. Link:http://ajpendo. physiology. org/content/294/6/E1088. full. pdf3. Title:p38 MAPK and LXR? mediate leptin effect on SREBP-1c expression in hepatic stellate cells (short title: Leptin effect on SREBP-1c expression) . Publication:Link:http://www. molmed. org/content/papers%20in%20press/11_243_Yan. pdf4. Title:Neuroprotection by leptin in a rat model of permanent cerebral ischemia: effects on STAT3 phosphorylation in discrete cells of the brain. Publication:Citation: Cell Death and Disease (2011) 2, e238; doi:10.1038/cddis. 2011. 125Published online 8 December 2011. Link:http://www. nature.com/cddis/journal/v2/n12/full/cddis2011125a. html 5. Title: The mammalian target of rapamycin as novel central regulator of puberty onset via modulation of hypothalamic Kiss1 system. Publication: Endocrinology 150.11 (2009): 5016-5026. Link http://press. endocrine. org/doi/full/10.1210/en. 2009-0096
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