Recombinant human TNF-a produced in HEK cells is a glycosylated non-disulfide linked homotrimer, containing 157 and having total Mw of 17 kDa. The TNF-a is purified by proprietary chromatographic techniques.
Synonyms: TNF-Alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Formulation: The TNF-a protein was lyophilized from 1mg/ml in 1xPBS.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as obsereved by SDS-PAGE.
Source: HEK.
Amino acid sequence: VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQ
LVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL.
Uniprot ACC number: P01375
Transportation method: Shipped at room temperature.
Stability: Lyophilized TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity was determined by the dose-dependent cytotoxity of the TNF Alpha sensitive cell line L-929 in the presence of Actinomycin D and is typically 0.05-0.5 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized TNF-a in sterile water not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com