Recombinant human TGFB3 produced in plant is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 118 amino acids and having a molecular mass of 27.2 kDa. The TGFB3 is fused to 6xHis-Tag at N-terminus and purified by standard chromatographic techniques.
Synonyms: Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
Formulation: lyophilized from a concentrated (1mg/ml) solution containing 50 mM Tris-HCl pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source: Nicotiana benthamiana.
Amino acid sequence: HHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS.
Uniprot ACC number: P106 00
Transportation method: Shipped at room temperature.
Stability: Lyophilized TGFB3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB3 Human should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The biological activity of TGFB3 is measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. ED50 ? 40 ng/ml corresponding to a specific activity of 25,000 Units/mg.
Solubility: It is recommended to reconstitute the lyophilized TGFB3 in sterile 5 mM HCl & 50ug/ml BSA at a concentration of 0.05mg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com