Recombinant human TGFB1 produced in CHO cells is a glycosylated homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25.6 kDa. The TGFB1 is purified by proprietary chromatographic techniques.
Synonyms: Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.
Formulation: lyophilized from a sterile filtered solution containing 0.1 % trifluoroacetic acid (TFA) and trehalose (1:20 protein to Trehalose ratio).
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source: CHO cells.
Amino acid sequence: ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.
Uniprot ACC number: P01137
Transportation method: Shipped at room temperature.
Stability: Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB1 Human should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependent inhibition of IL-4-induced proliferation of HT-2 cells is 0.148 ng/ml, corresponding to a specific activity of 6.8x106 units/mg.
Solubility: It is recommended to reconstitute the lyophilized TGFB1 in sterile 10 mM HCl at a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title: attenuates skeletal muscle dystropH y in mdx mice . Publication:Published online before print March 16, 2009, doi: 10.109 6/fj.09-129833 August 2009 The FASEB Journal vol.23 no.8 2539-2548 . Link:http://www. fasebj. org/content/23/8/2539. full2. Title:Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins. Publication:Kallio H, Tolvanen M, J?nis J, Pan P-w, Laurila E, et al. (2011) Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins. PLoS ONE 6(11): e27152. doi:10.1371/journal. pone.0027152Link:http://www. plosone. org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0027152 3. Title: Cancer-Associated Carbonic Anhydrases IX and XII: Effect of Growth Factors on Gene Expression in Human Cancer Cell Lines. Publication: Journal of Cancer Molecules 5(3): 73-78, 2010. Link: http://mupnet. com/JOCM%205(3)%2073-78. pdf
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