Recombinant human Neurotrophin-3 produced in
E. coli is a non-glycosylated and non-covalently linked homodimer, containing 2x119 amino acid chains, having a total Mw of 27.2 kDa. The NT-3 is purified by proprietary chromatographic techniques.
Synonyms: Neurotrophic factor, Nerve growth factor-2, NGF-2, HDNF, NT-3.
Formulation: lyophilized from 0.02% TFA.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: YAEHKSHRGE YSVCDSESLW VTDKSSAIDI RGHQVTVLGE IKTGNSPVKQ YFYETRCKEA RPVKNGCRGI DDKHWNSQCK TSQTYVRALT SENNKLVGWR WIRIDTSCVC ALSRKIGRT.
Uniprot ACC number: P20783
Transportation method: Shipped at room temperature.
Stability: Lyophilized NGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-2 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependent proliferation of C6 cells and is 3.6-5.4?g/ml.
Solubility: It is recommended to reconstitute the lyophilized Neurotrophin-3 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Generation of Neurons with Improved Cell Survival and pH enotype Maintenance Using a Degradation-Resistant Nurr1 Mutant[1]^†[2]^‡. Publication:Article first published online: 11 JUN 2009 DOI: 10.1002/stem.146 Copyright © 2009 AlphaMed Press. Link:http://onlinelibrary. wiley. com/doi/10.1002/stem.146/fullLinks:1. http://onlinelibrary. wiley. com/doi/10.1002/stem.146/full#fn12. http://onlinelibrary. wiley. com/doi/10.1002/stem.146/full#fn3 2. Title:Mash1 and Neurogenin 2 Enhance Survival and Differentiation of Neural Precursor Cells After Transplantation to Rat Brains via Distinct Modes of Action. Publication: Received 22 May 2008; Accepted 8 August 2008; Published online 9 September 2008. Molecular Therapy (2008) 16 11, 1873–1882 doi:10.1038/mt.2008.189Link:http://www. nature. com/mt/journal/v16/n11/full/mt2008189a. html 3. Title: TrkA Receptor “Hot Spots” for Binding of NT-3 as a Heterologous Ligand Publications: Journal of Biological Chemistry 282.23 (2007): 16754-16763. Link: http://www. jbc. org/content/282/23/16754. full 4. Title: OrpH an nuclear receptor Nurr1 induces neuron differentiation from embryonic cortical precursor cells via an extrinsic paracrine mechanismPublication: FEBS letters 583.9 (2009): 1505-1510Link http://www. sciencedirect. com/science/article/pii/S0014579309002828
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