Recombinant human Keratinocyte Growth Factor-1 produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 19 kDa. The FGF-7 is purified by proprietary chromatographic techniques.
Synonyms: HBGF-7, FGF7, FGF-7, KGF.
Formulation: lyophilized from a 0.2?m filtered solution in 20 mM PB, pH 8.0, 1M NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MCNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAIT.
Uniprot ACC number: P21781
Transportation method: Shipped at room temperature.
Stability: Lyophilized Keratinocyte Growth Factor1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF7 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The biological activity was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing KGF receptors yielding an ED50 <10 ng/ml, corresponding to a Specific Activity of 1.0×105 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Keratinocyte Growth Factor in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Human Cord Blood-Derived Endothelial Progenitor Cells and Their Conditioned Media Exhibit Therapeutic Equivalence for Diabetic Wound Healing . Publication: Cell Transplantation ISSN: 0963-6897 DOI: 10.3727/096368910X516637 Volume 19, Issue 12, pages 1635-1644 Copyright © 2010 Cognizant Co mM. CorpLink:http://www. ingentaconnect. com/content/cog/ct/2010/00000019/00000012/art000112. Title:Transforming Growth Factor-Alpha: A Major Human Serum Factor that Promotes Human Keratinocyte Migration. Publication:Journal of Investigative Dermatology (2006) 126, 2096–2105 . doi:10.1038/sj. jid.5700350; published online 11 May 2006. Link:http://www. nature. com/jid/journal/v126/n9/full/5700350a. html
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