Recombinant human Interleukin-3 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15 kDa. The IL-3 is purified by proprietary chromatographic techniques.
Synonyms: MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.
Formulation: lyophilized from a concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln.
Uniprot ACC number: P08700
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml
, corresponding to a Specific Activity of 10,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Interleukin-10 overexpression in Macrophages suppresses atherosclerosis in hyperlipidemic mice. Publication:Published online before print March 30, 2010, doi: 10.109 6/fj.09-148155 August 2010 The FASEB Journal vol.24 no.8 2869-2880 . Link:http://www. fasebj. org/content/24/8/2869. full2. Title:A Method for the Selection of Human Embryonic Stem Cell Sublines with High Replating Efficiency After Single-Cell Dissociation. Publication:Article first published online: 24 AUG 2006 DOI:10.1634/stemcells.2005-0657 Copyright © 2006 AlphaMed PressLink:http://onlinelibrary. wiley. com/doi/10.1634/stemcells.2005-0657/full3. Title: Selective Activation of Human Dendritic Cells by OM-85 through a NF-kB and MAPK Dependent PathwayPublications: PloS one 8.12 (2013): e82867Link: http://journals. plos. org/plosone/article?id=10.1371/journal. pone.0082867#pone-0082867-g0054. Title: Novel markers of normal and neoplastic human plasmacytoid dendritic cellsPublication: Blood 111.7 (2008): 3778-3792. Link: http://www. bloodjournal. org/content/111/7/3778
Safety data sheet: Inquire at info@3hbiomedical.com