Recombinant human Interleukin-2 produced in HEK cells is a glycosylated monomer, having a total molecular weight of 15 kDa. The IL2 is purified by proprietary chromatographic techniques.
Synonyms: T-cell growth factor (TCGF), Aldesleukin, LympH okine, IL-2.
Formulation: The IL2 was lyophilized from 0.2µm filtered solution containing 0.76mg/ml protein in 1xPBS.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as obsereved by SDS-PAGE.
Source: HEK.
Amino acid sequence: APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLE EELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT.
Uniprot ACC number: P60568
Transportation method: Shipped at room temperature.
Stability: Lyophilized IL-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity as determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line) was measured to be 1.57 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized IL-2 in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Safety data sheet: Inquire at info@3hbiomedical.com