Recombinant human Interleukin-2 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15.4 kDa. The IL-2 is purified by proprietary chromatographic techniques.
Synonyms: T-cell growth factor (TCGF), LympH okine, IL-2.
Formulation: The protein was lyophilized from a 0.2μm filtered concentrated solution (1.0mg/ml) in PBS, pH 5.0.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: APTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLT.
Uniprot ACC number: P60568
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL2 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is less than 0.1 ng/ml
, corresponding to a Specific Activity of 1x107 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Interleukin-2 in sterile distilled water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:CD8 Kinetically Promotes Ligand Binding to the T-Cell Antigen Receptor. Publication:BiopH ysical Journal Volume 89 September 2005 2121–2133. Link:http://www. ncbi. nlm. nih. gov/pmc/articles/PMC1366714/pdf/2121. pdf2. Title: Detection of expressed IL-32 in human stomach cancer using ELISA and i mMunostainingPublication: J Microbiol Biotechnol 18.9 (2008): 1606-1612. Link: http://www. jmb. or. kr/submission/Journal/018/JMB018-09-19. pdf3. Title: Conservation of cytokine-mediated responses in innate i mMunity: a flow cytometric study investigating the effects of human proInflammatory cytokines on pH agocytosis in the earthworm Eisenia hortensisPublication: Invertebrate Survival Journal 5 (2008): 124-134. Link: http://www. isj. unimo. it/articoli/ISJ175. pdf
Safety data sheet: Inquire at info@3hbiomedical.com