Recombinant human Interleukin-18 produced in
E. coli is a single, non-glycosylated polypeptide chain containing 157 amino acids and having a molecular mass of 18.2 kDa. The IL-18 is purified by proprietary chromatographic techniques.
Synonyms: IGIF, IL-1g, IL-18, IL1F4, MGC12320, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Iboctadekin.
Formulation: lyophilized from a concentrated (1mg/ml) solution in PBS and DTT.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by SDS-PAGE.
Source:Escherichia coli. Uniprot ACC number: Q14116
Transportation method: Shipped at room temperature.
Stability: Lyophilized Interleukin 18 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL18 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 is 1.5-9 ng/ml
and is measured by Interleukin 18 ability to induce IFN-γ secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-Alpha.
Solubility: It is recommended to reconstitute the lyophilized Interleukin 18 in sterile 18MΩ-cm H
2O not less than 200µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Discovery of IL-18 As a Novel Secreted Protein Contributing to Resistance by Comparative Secretome Analysis of MCF-7 and MCF-7/Dox. Publication:Yao L, Zhang Y, Chen K, Hu X, Xu LX (2011) Discovery of IL-18 As a Novel Secreted Protein Contributing to Resistance by Comparative Secretome Analysis of MCF-7 and MCF-7/Dox. PLoS ONE 6(9): e24684. doi:10.1371/journal. pone.0024684Link:http://www. plosone. org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0024684 2. Title: Influence of Interleukin IL-2 and IL-12 + IL-18 on Surface Expression of I mMunoglobulin-Like Receptors KIR2DL1, KIR2DL2, and KIR3DL2 in Natural Killer CellsPublications: Mediators of infla mMation 2006 (2006). Link: http://www. ncbi. nlm. nih. gov/pmc/articles/PMC1618942/
Safety data sheet: Inquire at info@3hbiomedical.com