Recombinant human IFN-gamma produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 17 kDa. The IFN-gamma is purified by proprietary chromatographic techniques.
Synonyms: Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
Formulation: The protein was lyophilized from a 0.2 µm filtered concentrated solution in PBS pH 4.6.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ.
Uniprot ACC number: P01579
Transportation method: Shipped at room temperature.
Stability: Lyophilized IFN gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity as determined in a viral resistance assay is < 0.05 ng/ml, corresponding to a specific activity of 2.0 x 10,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized IFN-gamma in sterile distilled water or 20 mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Candida albicans abrogates the expression of IFN-G-inducible protein-10 in human keratinocytes. Publication:Article first published online: 18 JUL 2008DOI: 10.1111/j.1574-695X.2008.00457. xLink:http://onlinelibrary. wiley. com/doi/10.1111/j.1574-695X.2008.00457. x/full 2. Title: Augments Cytokine-Induced Nitric Oxide Production in Human Alveolar Epithelial CellsPublications: Antimicrobial agents and chemotherapy 50.1 (2006): 396-398Link: http://aac. asm. org/content/50/1/396. full 3. Title: Selective Activation of Human Dendritic Cells by OM-85 through a NF-kB and MAPK Dependent PathwayPublications: PloS one 8.12 (2013): e82867Link: http://journals. plos. org/plosone/article?id=10.1371/journal. pone.0082867#pone-0082867-g005 4. Title: A New Strategy to Induce Effective Antitumour Response
in vitro and
in vivoPublications: Scandinavian journal of Immunology 68.3 (2008): 287-296Link: http://onlinelibrary. wiley. com/doi/10.1111/j.1365-3083.2008.02140. x/full 5. Title: Capture of Tumor Cell Membranes by Trogocytosis Facilitates Detection and Isolation of Tumor-Specific Functional CTLsPublication: Cancer research 68.6 (2008): 2006-2013. Link: http://cancerres. aacrjournals. org/content/68/6/2006. full 6. Title: Curcumin induces maturation-arrested dendritic cells that expand regulatory T cells
in vitro and
in vivoPublication: Clinical & Experimental Immunology 162.3 (2010): 460-473. Link: http://onlinelibrary. wiley. com/doi/10.1111/j.1365-2249.2010.04232. x/full
Safety data sheet: Inquire at info@3hbiomedical.com