Recombinant human Flt3-Ligand produced in
E. coli is a non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of approximately 17.6 kDa. Flt3-Ligand is purified by proprietary chromatographic techniques.
Synonyms: Fms-Related Tyrosine Kinase 3 Ligand, Flt3 Ligand, Flt-3 Ligand, SL Cytokine.
Formulation: The protein was Lyophilized with no additives.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA.
Uniprot ACC number: P49771
Transportation method: Shipped at room temperature.
Stability: Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-Ligand should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as calculated by the dose-dependent stimulation of the proliferation of human AML5 cells is less than 1.0 ng/ml, corresponding to a Specific Activity of 1.0×10
6 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Flt3-L in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: 1. Title:Identification of Y589 and Y599 in thejuxtamembrane domain of Flt3 as ligand-induced autophosphorylation sites involved in binding of Src family kinases and the protein tyrosine pH ospH atase SHP2. Publication:Published online before printMay 9, 2006, doi:10.1182/blood-2005-07-008896Submitted: July 14, 2005Accepted: April 26, 2006 BloodSeptember 1, 2006vol.108 no.51542-1550. Link:http://bloodjournal. hematologylibrary. org/content/108 /5/1542. full2. Title:A role of Gab2 association in Flt3 ITD mediated Stat5 phosphorylation and cell survival. Publication:Received 2 February 2009; accepted for publication 30 March 2009 Correspondence: Lars Ro¨nnstrand, Experimental Clinical Chemistry, WallenbergLaboratory, Entr.46, 5th fl, Malmo¨ University Hospital, SE-20502 Malmo¨, Sweden. E-mail: lars. ronnstrand@med. lu. se;http://www. expklkemi. mas. lu. seLink:http://onlinelibrary. wiley. com/doi/10.1111/j.1365-2141.2009.07725. x/pdf
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