Recombinant human Epidermal Growth Factor (1-51 a.a.) produced in yeast is a single, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 6.0 kDa. The EGF is purified by proprietary chromatographic techniques.
Synonyms: Urogastrone, URG, EGF.
Formulation: lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source: Saccharomyces cerevisiae
Amino acid sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW E.
Uniprot ACC number: P01133
Transportation method: Shipped at room temperature.
Stability: Lyophilized EGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Epidermal Growth Factor should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The ED50 is determined by a cell proliferation assay using murine Balb/c 3T3 cells and is < than 0.1 ng/ml
, corresponding to a specific activity of > 1.0 × 107 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H
2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com