Recombinant human Bone Morphogenetic Protein-7 produced in
E. coli is a monomeric, non-glycosylated, polypeptide chain containing 139 amino acids and having a molecular mass of 15.7 kDa. The BMP-7 is purified by proprietary chromatographic techniques.
Synonyms: Osteogenic Protein 1, BMP-7.
Formulation: BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10 mM sodium citrate pH 3.5.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Thr-Gly-Ser-Lys.
Uniprot ACC number: P18075
Transportation method: Shipped at room temperature.
Stability: Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP 7 Human should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Solubility: It is recommended to briefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in 20 mM-100 mM acetic acid at a concentration of 0.1-0.5mg per ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
References: 1. Title: Pulpal and periapical response of dogs' teeth after pulpotomy and use of recombinant human Bone Morphogenetic protein-7 as a capping agentPublications: J Dent Child 74 (2007): 79-84. Link: http://www. researchgate. net/profile/Francisco_Paula-Silva/publication/5374974_Pulpal_and_periapical_response_of_dogs'_teeth_after_pulpotomy_and_use_of_recombinant_human_bone_morpH ogenetic_protein-7_as_a_capping_agent/links/5413605e0cf2788c4b359755. pdf2. Title: Pretreatment with Bone Morphogenetic Protein-7 (BMP-7) Mimics Ischemia Preconditioning Following Intestinal Ischemia/Reperfusion Injury in The Intestine and LiverPublications: Shock (Augusta, Ga. )30.5 (2008): 532. Link: http://www. ncbi. nlm. nih. gov/pmc/articles/PMC3800102 /3. Title: BMP-7 blocks mesenchymal conversion of mesothelial cells and prevents peritoneal damage induced by dialysis fluid exposurePublication: NepH rology Dialysis Transplantation (2010): gfp618. Link: http://ndt. oxfordjournals. org/content/early/2010/01/18/ndt. gfp618. full
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