Recombinant human BMP-2 produced in HEK cells is a glycosylated disulfide-linked homodimer, having a molecular weight range of 28 kDa due to glycosylation. The BMP2 is purified by proprietary chromatographic techniques.
Synonyms: BMP-2, BMP2A.
Formulation: The BMP2 was lyophilized from 0.67mg/ml in 2xPBS + 6% ethanol.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by analysis by SDS-PAGE.
Source: HEK.
Amino acid sequence: QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNST NHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR.
Uniprot ACC number: P12643
Transportation method: Shipped at room temperature.
Stability: Lyophilized BMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP-2 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The specific activity as determined by the dose dependent induction of alkaline pH ospH atase production in the ATDC-5 cell line (Mouse chondrogenic cell line) was found to be 6.53 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized BMP-2 in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant HSA.
Safety data sheet: Inquire at info@3hbiomedical.com