Recombinant human BMP13 produced in
E. coli is a non-glycosylated disulfide linked homodimer containing 2 chains of 120 amino acids and having a molecular mass of 27.1 kDa. The BMP-13 is purified by proprietary chromatographic techniques.
Synonyms: Growth Differentiation Factor 6, Growth/Differentiation Factor 16, Bone Morphogenetic Protein 13, BMP-13, BMP13, GDF-6, Klippel-Feil Malformation, Segmentation Syndrome 1, Klip-Feil Malformation, Klippel-Feil Syndrome, MCOPCB6, SCDO4, CDMP2, LCA17, MCOP4, GDF16, KFS1, KFSL, SGM1, KFM, KFS, GDF6.
Formulation: BMP-13 protein was lyophilized from a 0.2 µm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: TAFASRHGKR HGKKSRLRCS KKPLHVNFKE LGWDDWIIAP LEYEAYHCEG VCDFPLRSHL EPTNHAIIQT LMNSMDPGST PPSCCVPTKL TPISILYIDA GNNVVYKQYE DMVVESCGCR.
Uniprot ACC number: Q6KF10
Transportation method: Shipped at room temperature.
Stability: Lyophilized BMP13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP-13 should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by inducing alkaline pH ospH atase production of murine ATDC5 cells is less than 2.0 µg/ml, corresponding to a specific activity of > 500IU/mg.
Solubility: It is recommended to reconstitute the lyophilized BMP13 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com