Recombinant human BAFF produced in
E. coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17 kDa. The BAFF is purified by proprietary chromatographic techniques.
Synonyms: BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, TNFSF13B, B-cell Activating Factor.
Formulation: lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.0.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 95% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli. Amino acid sequence: MAVQGPEETV TQDCLQLIAD SETPTIQKGS YTFVPWLLSF KRGSALEEKE NKILVKETGY FFIYGQVLYT DKTYAMGHLI QRKKVHVFGD ELSLVTLFRC IQNMPETLPN NSCYSAGIAK LEEGDELQLA IPRENAQISL DGDVTFFGAL KLL.
Uniprot ACC number: Q9Y275
Transportation method: Shipped at room temperature.
Stability: Lyophilized BAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BAFF should be stored at +4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Biological Activity: The activity is determined by a mouse splenocyte survival assay. The ED50 for this effect is 0.5-2.0 µg/ml.
Solubility: It is recommended to reconstitute the lyophilized BAFF in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Safety data sheet: Inquire at info@3hbiomedical.com