GRO Alpha Human Recombinant produced in
E. coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7.8 kDa. The GRO-alpha is purified by proprietary chromatographic techniques.
Synonyms: Growth-regulated protein alpha, CXCL1, Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3, GRO-alpha (1-73), chemokine (C-X-C motif) ligand 1, GRO1, GROa, SCYB1, MGSA-a, MGSA alpha.
Formulation: Lyophilized from a 0.2 µm filtered concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 50mM NaCl.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ser-Val-Ala-Thr.
Uniprot ACC number: P09341
Transportation method: Shipped at room temperature.
Stability: Lyophilized GRO-alpha although stable at room temperature.erature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CXCL1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity: Determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 10.0-100.0 ng/ml.
Solubility: It is recommended to reconstitute the lyophilized CXCL1 in sterile 18MΩ-cm H
2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: Title:Deficient inflammatory response to UV radiation in neonatal mice.Publication:Published online before print March 16, 2007, doi: 10.1189/jlb.1206729 June 2007 Journal of Leukocyte Biology vol. 81 no. 6 1352-1361 Link:http://www.jleukbio.org/content/81/6/1352.full
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