Parathyroid Hormone (C181H290N55O51S2) produced in
E. coli is a single, non-glycosylated, polypeptide chain containing 34 amino acids and having a molecular mass of 4.1 kDa. The PTH is purified by proprietary chromatographic techniques.
Synonyms: Parathyrin, PTH, Parathormone.
Formulation: The protein (1 mg/ml) was lyophilized after extensive dialyses against 1.15 mg sodium citrate, sodium chloride 7.31 mg, 0.21 mg citric acid, 0.1117 EDTA-Na2, 0.2 mg Tween 80 and 50 mg Mannitol.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.Amino acid sequence: Ser-Val-Ser-Glu-Ile-Gln-Leu-Met-His-Asn-Leu-Gly-Lys-His-Leu-Asn-Ser-Met-Glu-Arg-Val-Glu-Trp-Leu-Arg-Lys-Lys-Leu-Gln-Asp-Val-His-Asn-Phe.
Uniprot ACC number: P01270
Transportation method: Shipped at room temperature.
Stability: Lyophilized Parathyrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTH should be stored at +4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity: The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 10,000 Units/mg.
Solubility: It is recommended to reconstitute the lyophilized Parathormone in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
References: Title:Cathepsin K Expression and Activity in Canine Osteosarcoma. Publication: Article first published online: 15 DEC 2011 DOI:10.1111/j.1939-1676.2011.00834.x Copyright © 2011 by the American College of Veterinary Internal Medicine. Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1939-1676.2011.00834.x/full
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