(Click to display a larger image)
Protein Purification
Catalog number: ABMG250
Unit size: 25 ml
Affinity chromatography is a method of separating biochemical mixtures based on highly specific molecular interactions. The Ni-IDA agarose is developed for the purification of proteins with an affinity tag of six consecutive histidine residues. This histidine-metal ion interaction makes one-step purification possible for proteins from any expression system, under native or denaturing conditions. The His-tag sequence binds to Ni2+ cations, which are immobilized onto a solid support using iminodiacetic acid groups (IDA). Impurities are removed and the purified His-tagged proteins can be eluted by imidazole or a reduced pH solution.
Article number:ABMG250
Unit:pcs
Stock status:Special Order Item
Special Features:
- One-step easy purification protocol from crude lysate to >95% pure protein.
- Purification under native or denaturing conditions.
- Ready-to-use for any scale of purification.
- Protein binding capacity: approx. 20-40 μmol Ni2+/ml gel.
- Economical value.
- Can be used for successive cycles.
- Resins and columns formats suitable for batch and gravity purification.
Application:
- Purification of His-tagged proteins.
- Antibody purification.
- Assays involving protein-protein and protein-DNA interactions.
- Structural and functional investigation of proteins.
Storage Condition:
Resin should be stored at +2-+8°C. Do not freeze.