This 2nd generation lentiviral packaging mix replaces the envelope protein Vesicular Stomatitis Virus (VSV) glycoprotein (G) with the Spike (S) Protein from SARS-CoV-2.
For the production of lentiviral particles, three components are generally required: 1) a lentiviral vector containing your inserts of interest (cDNA, shRNA, or miRNA), 2) one or two packaging vectors which contain all necessary viral structure proteins, 3) an envelope vector. In general, lentiviral vectors with a wildtype 5' LTR need the 2nd generation packaging system because these vectors require TAT for activation. However, the 2nd generation packaging mix will also support the production of 3rd generation lentiviral expression vector with a chimeric 5' LTR. All the lentiviral expression vectors marketed by abm are 3rd generation with a chimeric of RSV promoter upstream of 5'-LTR.
Manufacturer
ABM (Applied Biological Materials)
Product Link
LV700
Storage
-20°C
Shipping
Ambient Temperature