Quality Control: Each batch of TCF/LEF Dual Reporter Vector (TDRV) undergoes stringent quality control to ensure optimal performance and reliability. Plasmid DNA purity is confirmed by spectrophotometric analysis (A260/A280 ratio between 1.7 and 2.1), while integrity and size of plasmids are verified by agarose gel electrophoresis. The sequences of key regulatory elements and luciferase genes are validated by Sanger sequencing, and functional activity is confirmed by transient transfection and treatment (LiCl 10mM) in HEK293T, followed by Luciferase Assay Kit (LAK, Cat no. SC9048) to verify expected Firefly and Renilla luminescence signals (Figure 1). This rigorous quality control guarantees reproducible and reliable results in your gene expression and signaling pathway studies.
Product Use: TDRV is for research use only and is not approved for human or animal use, or application in clinical or in vitro diagnostic procedures.
Shipping and Storage: The product is shipped at room temperature. Upon receipt, store at -20°C.
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[2] Brennan, K., et al. (2004). Inhibition of Wnt/β-catenin signaling by a soluble receptor-1 in a colorectal cancer model. Cancer Research, 64(13), 5034-5042
[3] Thorne, C. A., et al. (2010). Small-molecule inhibition