Origin of cells

Human Pan T cells were isolated from normal human peripheral blood mononuclear cells of a single donor.

Human Pan T cells are > 90% CD3 positive cells.

Total RNA preparation

Human Pan T cell total RNA was prepared using a modified guanidium method and treated with RNase-free DNase. 

cDNA preparation

First strand cDNA was synthesized from 1-2 microgram of Human Pan T cell total RNA using oligonucleotides random primers and reverse-transcribed by reverse transcriptase in 20 µl final volume. The RT reaction was stopped by heating. 1 µl cDNA is sufficient for one PCR reaction.

Certificate of analysis

The cDNA has been performance-tested by PCR amplification using GAPDH primers.

Applications

• Immediate PCR amplification of known genes
• Comparison of gene expression patterns between biological samples
• Gene mutation analysis
• Analysis of mRNA alternative splicing
• Gene cloning and target sequencing

Storage condition

-80°C.

Avoid repeated freeze/thaw cycles. 

Shipping condition 

Dry-ice