Each primer set included in GQR-CE qPCR kit arrives lyophilized in a 2 mL vial. All primers are designed and tested under the same parameters: (i) an optimal annealing temperature of +65°C (with 2 mM Mg2+, and no DMSO); (ii) recognition of all known target gene transcript variants; and (iii) specific amplification of only one amplicon. Each primer set has been validated by qPCR by melt curve analysis and gel electrophoresis.
Quality Control: Each primer set is validated by qPCR melt curve and amplification curve analyses. The PCR products are analyzed by gel electrophoresis to confirm single band amplification.
Product Use: GQR-CE is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures. Shipping and Storage This product is shipped at ambient temperature. Upon receipt, the vials should be stored at +4°C and are good for up to 12 months. For long-term storage (>1 year), store the vials at -20°C in a manual defrost freezer.