Each primer set included in GQP-CE qPCR kit arrives lyophilized in a 2 mL vial. All primers are designed and tested under the same parameters: (i) an optimal annealing temperature of +65°C (with 2 mM Mg2+, and no DMSO); (ii) recognition of all known target gene transcript variants; and (iii) specific amplification of only one amplicon. Each primer set has been validated by qPCR by melt curve analysis and gel electrophoresis.
Quality Control: Each primer set is validated by qPCR melt curve and amplification curve analyses. The PCR products are analyzed by gel electrophoresis to confirm single band amplification.
Product Use: GQR-CE is for research use only. It is not approved for human or animal use or for application in clinical or in vitro diagnostic procedures.
Shipping and Storage: This product is shipped at ambient temperature. Upon receipt, the vials should be stored at +4°C and are good for up to 12 months. For long-term storage (>1 year), store the vials at -20°C in a manual defrost freezer.