Quality control: Fibronectin quality is assessed with a NuPAGE 4-12 % Bis-Tris Gel stained with Coomassie brilliant blue. Under reducing conditions, fibronectin appears as a doublet of 230 and 220 kDa. ELISA assays show that absorbance is directly proportional to the logarithm of fibronectin concentration. Cell adhesion assays indicate that a coating with as low as 0.1 μg/cm2 of fibronectin significantly promotes endothelial cell adhesion compared with non-coated controls.
Storage/Handling: It is recommended to store the product as single use aliquots at -80 ̊C. Thawing should be done slowly at 2- 8°C with no agitation. Material that fails to dissolve can be removed by centrifugation. Avoid repeated freeze/thaw cycles.
Application: Recommended for use as a cell culture substratum at 1-5 μg/cm2. Optimal concentration depends on cell type.