Heparin (HEP) is a 10-12 kDa polysaccharide produced by mast cells. Heparin inhibits blood coagulation through binding and activation of anti-thrombin and acts as a cofactor to basic Fibroblast Growth Factor-2.
The effect of heparin on cell growth and morphology is cell-type dependent. Heparin may promote or inhibit cell proliferation.
0,2% Heparin sodium salt in DPBS solution can be used as additive to cell culture media at dilution of 1:1000-1:100 (0.4 – 4.0 U/ml). The optimal concentration should be determined for individual cell types and culture conditions.
Concentration
2 mg/ml heparin sodium salt (from porcine intestinal mucosa) in Dulbecco’s Phosphate-Buffered Saline, sterile-filtered.
Potency
200 Units per milligram*.
*Units are USP (United States Pharmacopeia units) and indicate heparin potency by weight.
Product Use
HEP is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.
Storage
Store at 4°C.
Shipping
Gel ice.