Vitronectin is involved in a number of biological functions including cell adhesion, spreading, migration, proliferation, extracellular anchoring, fibrinolysis, hemostasis, and complement immune defense [2, 3, 4]. Vitronectin can be used for coating tissue culture surfaces to promote cell adhesion, proliferation, and differentiation.
ScienCell™ Bovine Plasma Vitronectin is purified from bovine plasma by affinity chromatography [5]. It is supplied as a sterile solution in Dulbecco's Phosphate-Buffered Saline(DPBS). Optimal conditions for cell attachment must be determined for each cell line and application.
Product Specification:Quantity: 100 μg. Concentration: 0.4 mg/ml. Storage buffer: DPBS, pH 7.4.
Quality Control: Vitronectin quality is assessed by NuPAGE 4-12% Bis-Tris Gel stained with Coomassie brilliant blue. Under reducing conditions, vitronectin appears as a doublet with bands at 75 kDa and 65 kDa. Cell adhesion assays indicate that coating at as low as 0.1 μg/cm2 of vitronectin promotes endothelial cell adhesion compared to non-coated controls.
Storage/Handling: It is recommended that the product be aliquoted and stored at -80 ̊C. Vitronectin should be thawed slowly at 2-8°C with no agitation. Any precipitate that is present can be removed by centrifugation. Avoid repeated freeze/thaw cycles.
References: [1] Vuento M, Korkolainen M, Kuusela P, Holtta E. (1985) "Isolation of a novel cell-attachment and spreading-promoting protein from human serum." Biochem J. 227: 421-7. [2] Hayman EG, Pierschbacher MD, Suzuki S, Ruoslahti E. (1985) "Vitronectin--a major cell attachment promoting protein in fetal bovine serum." Exp Cell Res. 160: 245-58.[3] Tschopp J, Masson D, Schafer S, Peitsch M, Preissner KT. (1988) "The heparin binding domain of S protein/vitronectin binds to complement components C7, C8, and C9 and perforin from cytolytic T cells and inhibits their lytic activities." Biochemistry. 27:4103-9. [4] Yatohgo T, Izumi M, Kashiwagi H, Hayashi M. (1988) "Novel purification of vitronectin from human plasma by heparin affinity chromatography." Cell Struct Funct. 13:281-92.[5] Akiyama KS. (1999) "Purification of Vitronectin." Current Protocols in Cell Biology. 10.6.1-10.6.5