Product Use: SF-CFM is used to cryopreserve human and animal neurons. It is for research use only.
Not for use in animals, humans, or diagnostic procedures.
Storage: Store SF-CFM at -20°C. Once thawed, the product may be stored at 4°C for up to one month.
Shipping: Dry ice.
Procedure: Cryopreservation may compromise cell quality and performance. Performance of the cells cannot be guaranteed after cryopreservation. 1) Harvest and spin down cells. 2) Resuspend cells in cold SF-CFM at a density of ~500,000 to 2,000,000 cells/ml. Work quickly; once exposed to DMSO, cells become very fragile. 3) Pipet 1 ml into each freezing vial and tighten the cap. 4) Place the cells (vials) in a Styrofoam® or propanol freezing canister and store them at -80ºC overnight. 5) Within 12-24 hours, transfer the cells into LN2 freezer for long-term storage. Cells will be compromised by prolonged storage at -80ºC.
Caution: If handled improperly, some components of this product may present a health hazard. Take appropriate precautions when handling this product, including the wearing of protective clothing and eyewear. Dispose of properly.