Product Use: CFM is used to cryopreserve most human and animal cells. It is for research use only. Not for
use in animals, humans, or diagnostic procedures.
Storage: Store the CFM at -20°C. Once thawed, the product may be stored at 4°C for up to one month.
Shipping: Dry ice.
Procedure: Cryopreservation may compromise cell quality and performance. Performance of the cells cannot
be guaranteed after cryopreservation.
1) Harvest cells and spin them down.
2) Resuspend cells in cold CFM at a density of ~500,000 to 2,000,000 cells/ml. Work
quickly; once exposed to DMSO, cells become very fragile.
3) Pipet 1 ml into each freezing vial and tighten the cap.
4) Place the cells (vials) in a Styrofoam® or propanol freezing canister and store them at
-80ºC overnight.
5) Within 12-24 hours, transfer the cells into LN2 freezer for long-term storage. Cells will be
compromised by prolonged storage at -80ºC.
Caution: If handled improperly, some components of this product may present a health hazard.
Take appropriate precautions when handling this product, including the wearing of protective
clothing and eyewear. Dispose of properly.