Product Use: CFM is used to cryopreserve most human and animal cells. It is for research use only. Not foruse in animals, humans, or diagnostic procedures.
Storage: Store the CFM at -20°C. Once thawed, the product may be stored at 4°C for up to one month.
Shipping: Dry ice.
Procedure: Cryopreservation may compromise cell quality and performance. Performance of the cells cannotbe guaranteed after cryopreservation.1) Harvest cells and spin them down.2) Resuspend cells in cold CFM at a density of ~500,000 to 2,000,000 cells/ml. Workquickly; once exposed to DMSO, cells become very fragile.3) Pipet 1 ml into each freezing vial and tighten the cap.4) Place the cells (vials) in a Styrofoam® or propanol freezing canister and store them at-80ºC overnight.5) Within 12-24 hours, transfer the cells into LN2 freezer for long-term storage. Cells will becompromised by prolonged storage at -80ºC.
Caution: If handled improperly, some components of this product may present a health hazard.Take appropriate precautions when handling this product, including the wearing of protectiveclothing and eyewear. Dispose of properly.