Oligosphere Medium (OsM) is a complete medium designed for optimal growth of normal human oligospheres in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and factors prevent oligospheres attachment during culture. The medium is serum-free. It is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes growth of normal human oligospheres in vitro. This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.