Cardiac Myocyte Medium-serum free (CMM-sf), when used with Cardiac Myocyte Growth Supplement-serum free (CMGS-sf, Cat. no. 6152) is a complete medium designed for the culture of normal cardiac myocytes in serum-free conditions. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the culture of normal cardiac myocytes in vitro under serum free conditions. This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.