HCoSMC are isolated from the human colon. HCoSMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HCoSMC are characterized by immunofluorescence with antibodies specific to α- smooth muscle actin and desmin. HCoSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoSMC are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium: It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. no. SC1101) for culturing HCoSMC in vitro.
Product Use: HCoSMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping: Dry ice.