Tonsil epithelium is a heterogeneous lymphoid organ containing two different types of epithelia, the lining stratified squamous epithelium and the reticulated crypt, or lymphoepithelium.
The tonsil crypts represent a specialized compartment, important in the immunological functions of the tonsil because of the immediate proximity of the epithelial and lymphoid tissues. In situ staining of tonsil epithelium suggests that expression of the simple epithelial keratins K8, K18 and K19 is located to the crypt epithelial cells. Cell culture of tonsil epithelium produces heterogeneous squamous epithelial cells in which differentiation appears to be ongoing and keratin expression patterns are consistent with those found in tonsil epithelial cells in situ.
Human Tonsil Epithelial Cells (HTEpiC) are isolated from human tonsil. HTEpiC are cryopreserved at passage one and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HTEpiC are characterized by immunofluorescence with antibodies specific to Cytokeratine.
HTEpiC are guaranteed to further expand for 15 population doublings under the conditions provided.
It is recommended to use Tonsil Epithelial Cell Medium (TEpiCM, Cat. No. SC2561) for culturing HTEpiC in vitro.
HTEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.