HMEEpiC from ScienCell Research Laboratories are isolated from human middle ear. HMEEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HMEEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and cytokeratin-19. HMEEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HMEEpiC are guaranteed to further expand for 15 population doublings under the conditions provided.
Recommended Medium: It is recommended to use Epithelial Cell Medium-2 (EpiCM-2, Cat. no. SC4121) for culturing HMEEpiC in vitro.
Product Use: HMEEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage: Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen, and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping:Dry ice.
References: [1] Mulay A, Akram K, Williams D, Armes H, Russell C, Hood D, Armstrong S, Stewart J, Brown S, Bingle L, Bingle C. (2016) "An in vitro model of murine middle ear epithelium." Dis Model Mech. 9: 1405-1417.
[2] Straetemans M, van Heerbeek N, Tonnaer E, Ingels K, Rijkers G, Zielhuis G. (2001) "A comprehensive model for the aetiology of otitis media with effusion." Med Hypotheses. 57: 784-791.
[3] Xie S, Xiang Y, Wang X, Ren H, Yin T, Ren J, Liu W. (2016) "Acquired cholesteatoma epithelial hyperproliferation: Roles of cell proliferation signal pathways" Laryngoscope 126(8): 1923-1930.
[4] Yoshikawa, M, Kojima H, Yaguchi Y, Okada N, Saito H, Moriyama H. (2013) "Cholesteatoma fibroblasts promote epithelial cell proliferation through overexpression of epiregulin." PLoS One. 8(6): e66725.