The epidermal layer of the skin provides an essential function as a protective barrier against insults from the external environment. The predominant cell type in the epidermis is keratinocytes and they are located in the stratified squamous epithelia. Keratinocytes are named after keratin, which is the most abundant protein in this cell.
Human Epidermal Keratinocytes-adult (HEK-a) are isolated from adult human skin. HEK-a are cryopreserved at P0 and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HEK-a are characterized by immunofluorescence with antibodies specific to cytokeratine-18 and cytokeratine-19.
HEK-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HEK-a are guaranteed to further expand for 10 population doublings under the conditions provided.
Recommended Medium
It is recommended to use Keratinocyte Medium (KM, Cat. No. SC2101) for culturing HEK-a in vitro.
Storage
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.
Shipping
Dry ice.
Product Use
The commercial product is a tool for in vitro laboratory science research use only. NOT for human or animal diagnostics, therapeutic, or other clinical uses. NOT for household, or other uses.