The choroid plexus is located in the ventricles of the brain where cerebrospinal fluid (CSF) is constantly produced. It consists of a network of capillaries enclosed by a single layer of epithelial cells that together form the blood-CSF barrier.
Choroid plexus epithelial cells (CPEpiC) play a central role in restricting the passage of molecules and ions between the brain and CSF by the junctional complexes between neighboring cells. They also have a special cellular structure, and channel and transporter protein expression well-adapted for CSF secretion.
Human Choroid Plexus Epithelial Cells (HCPEpiC) are isolated from human choroid plexus. HCPEpiC are cryopreserved after purification and delivered frozen.
Each vial contains 500 000 cells in 1 ml volume.
HCPEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18, -19 and vimentin.
HCPEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
HCPEpiC are guaranteed to further expand for 5 population doublings under the conditions provided.
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. SC4101) for culturing HCPEpiC in vitro.
HCPEpiC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.
Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments.