Human Pan T cells were isolated from normal human peripheral blood mononuclear cells of a single donor.
Human Pan T cells are > 90% CD3 positive cells.
Human Pan T cell total RNA was prepared using a modified guanidium method and treated with RNase-free DNase.
First strand cDNA was synthesized from 1-2 microgram of Human Pan T cell total RNA using oligonucleotides random primers and reverse-transcribed by reverse transcriptase in 20 µl final volume. The RT reaction was stopped by heating. 1 µl cDNA is sufficient for one PCR reaction.
Certificate of analysis
The cDNA has been performance-tested by PCR amplification using GAPDH primers.
Avoid repeated freeze/thaw cycles.