Granulocytes are produced in the bone marrow and short-lived. Granulocytes represent about 60% to 70% of the total normal blood leuocytes.
Human Peripheral Blood Neutrophils represent over 90% of the circulating granulocytes.
Neutorphils have characteristic polymorphonuclear shap.
Neutrophils play key roles in the innte immune response and inflammatory response. Neutrophils migrate from the circulating peripheral blood to infected tissues in response to inflammatory stimuli. Neutrophils protect the host by phagocytotic activities, killing infectious microorganisms of bacterial and fungal.
Studies showed chronic neutrophilic inflammation in the initiation phase of many types of epithelial cancers and in the pathogenesis of the later phases of cancer development, specifically progression and spread (REF).
Fresh Human Peripheral Blood Neutrophils from a single donor are isolated from Buffy-Coat and deliver in Neutrophil culture medium.
Fresh Human Peripheral Blood Neutrophils from a single donor are isolated from Buffy-Coat and deliver in Neutrophil culture medium. Human Neutrophils express CD11b, CD16, and CD66b as cell surface markers. The purity of CD16 positive human Neutrophils is >98%.
The blood donor has been tested and found negative for HBsAG, HIV1/2, HIV-1 AG or HIV-1 NAT, HCV and syphilis.
Role of the Neutrophil in the Pathogenesis of Advanced Cancer and Impaired Responsiveness to Therapy
Bernardo L Rapoport, Helen C Steel, Annette J Theron, Teresa Smit, Ronald Anderson. Molecules. 2020 Apr 1;25(7):1618. doi: 10.3390/molecules25071618.